منابع مشابه
Human corticotropin (ACTH) radioimmunoassay with synthetic 1--24 ACTH.
A corticotropin antiserum was obtained from rabbits immunized with synthetic 1--24 corticotropin conjugated with bovine serum albumin. The antiserum did not cross react with synthetic alpha-melanotropin or with synthetic beta-endorphin and had a cross reactivity of 0.23% with human beta-lipotropin. We developed a radioimmunoassay with the antiserum obtained, in which we used polyethylene glycol...
متن کاملCytochemical bioassay and radioimmunoassay of ACTH in noise stressed rats.
Serum corticosterone and ACTH levels were measured in rats subjected to one and two 30 min intervals of noise (100 dB, 250--20,000 Hz). Both hormones were elevated immediately after noise exposure, dropped below pre-exposure levels after a 2 hr recovery interval and were again elevated with a second exposure. The ratio of immunoactive to bioactive ACTH was slightly depressed after one exposure ...
متن کامل[Radioimmunoassay of plasma cortisol].
A radioimmunoassay for the determination of plasma cortisol is described. The antiserum against cortisol was produced by the immunization of rabbits with cortisol21-BSA synthesized from cortisol via hemisuccinate derivative. The antiserum obtained had high affinity and high specificity for cortisol. 0.002 ml of plasma diluted with water was used for the assay. After 1,000 dpm of tritiated corti...
متن کاملRadioimmunoassay of cholecystokinin in plasma.
A sensitive and specific radioimmunoassay for cholecystokinin (CCK) has been developed. Synthetic unsulfated carboxy-terminal fragment, CCK-8, was radioiodinated by the conventional Chloramine-T method. Antibodies were raised against sulfated CCK-8 covalently coupled to bovine thyroglobulin via 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide. By purification, highly immunoreactive 125I-labeled CC...
متن کاملRadioimmunoassay for dexamethasone in plasma.
Until recently there has been no adequate clinical method for precisely measuring dexamethasone in blood. Studies of therapeutic dexamethasone concentrations and of its metabolism in plasma have relied on the use of radioactive dexamethasone (1-3). We report here an immunoassay in which antisera obtained from rabbits immunized with a conjugate of dexamethasone and bovine serum albumin (BSA) are...
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ژورنال
عنوان ژورنال: Journal of Clinical Investigation
سال: 1968
ISSN: 0021-9738
DOI: 10.1172/jci105955